career profile

 

graduate student with extensive research accomplishments in biotechnology and molecular biology within health science center and cancer research institute environments. successfully published research findings 3 times. desire to obtain a technician, lab manager, or research associate position.

education

 

university of texas, san antonio, tx, master of science in biotechnology, current

chinese academy of sciences, china, master of science in molecular biology, candidate

shandong agriculture university, china, bachelor of science in biotechnology, 2003

research

 

university of texas, san antonio, tx                                                                       2008-present

graduate student, health science center

     dna: plasmid manipulation (construction, verification, amplification of plasmids ), retroviral vector system, adenoviral vector system

     rna : rna isolation, reverse transcription, qpcr, northern

     protein: western, immunofluorescence assay (ifa), elisa, ip, in vitro translation, in vitro binding assay, gst protein preparation and purification   

 

greehey children s cancer research institute, san antonio, tx                        2006-2008

research scholar            

     tissue culture: 1) routine maintenance of cell culture, including hek293, 293t, ad-293, hela, huvecs (human umbilical vein endothelial cells), bcbl1, bcp1, bjab, telomerase immortalized dermal microvascular endothelial (time) cells, hct116, breast cancer cell lines, adipose stromal cells. 2) transfection, luciferase reporter assay

     dna methylation assay: bisulfite conversion of genomic dna, pcr, ta clone, sequencing, sequence analysis

     virus manipulation: amplification and infection of retrovirus, adenovirus and kaposi s sarcoma-associated herpesvirus, plaque assay of adenovirus, titration of retrovirus, adenovirus and kaposi s sarcoma-associated herpesvirus

publications

1) fang q, liu j, bai z q, kang t, he z h, hu z h, gao s-j*. seroprevalence of   kaposi s sarcoma-associated herpesvirus in the general population from hubei province. virologica sinica, 2006, 21 (2 ):97-101
2) ye fc, zhou fc, xie jp, kang t, greene w, kuhne k, lei xf, li qh, gao sj. kaposi's sarcoma-associated herpesvirus latent gene vflip inhibits viral lytic replication through nf-kappab-mediated suppression of the ap-1 pathway: a novel mechanism of virus control of latency. j.virol 2008 may;82(9):4235-49.
3) kang t, ye f c, gao s j, wang l d. angiogenesis, kaposi s sarcoma and kaposi s sarcoma-associated herpesvirus. virologica sinica, 2008, 23 (6): 449-458
4) fengchun ye, fuchun zhou, roble bedolla, tiffany jones, xiufen lei, yyyyyy x. yyyyyy, moraima guadalupe, shou-jiang gao. reactive oxygen species hydrogen peroxide induces kaposi's sarcoma-associated herpesvirus reactivation from latency (submitted to plos pathogens, 2010)
5) yunzhe lu, yyyyyy x. yyyyyy, rong li, and yanfen hu. brca1/bard1 complex interacts with steroidogenic factor 1----a potential mechanism for regulation of aromatase expression by brca1 (submitted to journal of cellular biochemistry, 2010 ).
6) yunzhe lu, yyyyyy x. yyyyyy, rong li, and yanfen hu.identification of a novel ubiquitin e3 ligase for brca1 (manuscript in preparation)
7) marc walter,yyyyyy x. yyyyyy, rong li, and yanfen hu.regulation of aromatase pii promoter by dna methylation
8) sagar ghosh, yyyyyy x. yyyyyy, yanfen hu, and rong li. collagen induces aromatase expression in adipose stromal cells (manuscript in preparation)

 

 

 

 

 

date

 

 

 

hiring agent name

title

company name

address

city/state/zip code

 

dear__________________:

 

i am currently seeking a challenging career opportunity in a technician, lab manager, or research associate capacity and am submitting my resume for your review. in advance, thank you for your time and consideration.

 

as demonstrated in the accompanying resume, my professional qualifications include more than 4 years of research experience in areas of biotechnology with the university of texas at san antonio. during this experience, i have effectively applied my analytical skills and advanced knowledge of biotechnology toward research to benefit the health sciences center and the greehey children s cancer research institute.

 

to complement this background, i am a candidate for a master of science in biotechnology and have successfully published research in academic journals and industry articles 3 times.

 

as an employee, you will find me to be a driven team player committed to supporting you in achieving your objectives through superior performance. i am confident that i could be a valuable asset to your firm, and look forward to interviewing with you in the near future.

 

sincerely,

 

 

yyyyyy x. yyyyyy

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

date

 

 

 

contact name

company name

address

city/state/zip code

 

dear _______________________________:

 

i would like to thank you for providing me the opportunity to interview with you and discuss the exciting (title of position applying for) position currently available with your company. having gained a deeper insight into the job requirements and your objectives, i am confident you will find me a valuable asset in achieving your goals.

 

as discussed, i am an enthusiastic, disciplined team player eager to launch a successful career as a dedicated member of your staff. i believe you will find my dynamic research and analytical skills as well as advanced knowledge of biotechnology applications in areas of health care and cancer research vital to your operations and look forward to contributing to your success.

 

should you have any additional questions, or to discuss future employment opportunities, please feel free to contact me at your earliest convenience. again, thank you for the enlightening interview. i look forward to your response.

 

sincerely,

 

 

yyyyyy x. yyyyyy