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yyyyyy x. yyyyyy
14825 omicron drive, #704 ~ san antonio, tx 78245 ~ (xxx-xxx-xxxx ~ abc@xyz.com
career profile
graduate student with extensive research accomplishments in biotechnology and molecular biology within health science center and cancer research institute environments. successfully published research findings 3 times. desire to obtain a technician, lab manager, or research associate position.
education
university of texas, san antonio, tx, master of science in biotechnology, current
chinese academy of sciences, china, master of science in molecular biology, candidate
shandong agriculture university, china, bachelor of science in biotechnology, 2003
research
university of texas, san antonio, tx 2008-present
graduate student, health science center
dna: plasmid manipulation (construction, verification, amplification of plasmids ), retroviral vector system, adenoviral vector system
rna : rna isolation, reverse transcription, qpcr, northern
protein: western, immunofluorescence assay (ifa), elisa, ip, in vitro translation, in vitro binding assay, gst protein preparation and purification
greehey children s cancer research institute, san antonio, tx 2006-2008
research scholar
tissue culture: 1) routine maintenance of cell culture, including hek293, 293t, ad-293, hela, huvecs (human umbilical vein endothelial cells), bcbl1, bcp1, bjab, telomerase immortalized dermal microvascular endothelial (time) cells, hct116, breast cancer cell lines, adipose stromal cells. 2) transfection, luciferase reporter assay
dna methylation assay: bisulfite conversion of genomic dna, pcr, ta clone, sequencing, sequence analysis
virus manipulation: amplification and infection of retrovirus, adenovirus and kaposi s sarcoma-associated herpesvirus, plaque assay of adenovirus, titration of retrovirus, adenovirus and kaposi s sarcoma-associated herpesvirus
publications
1) fang q, liu j, bai z q, kang t, he z h, hu z h, gao s-j*. seroprevalence of kaposi s
sarcoma-associated herpesvirus in the general population from hubei province.
virologica sinica, 2006, 21 (2 ):97-101
2) ye fc, zhou fc, xie jp, kang t, greene w, kuhne k, lei xf, li qh, gao
sj. kaposi's sarcoma-associated herpesvirus latent gene vflip inhibits viral
lytic replication through nf-kappab-mediated suppression of the ap-1 pathway: a
novel mechanism of virus control of latency. j.virol 2008 may;82(9):4235-49.
3) kang t, ye f c, gao s j, wang l d. angiogenesis, kaposi s sarcoma and
kaposi s sarcoma-associated herpesvirus. virologica sinica, 2008, 23 (6):
449-458
4) fengchun ye, fuchun zhou, roble bedolla, tiffany jones, xiufen lei, yyyyyy
x. yyyyyy, moraima guadalupe, shou-jiang gao. reactive oxygen
species hydrogen peroxide induces kaposi's sarcoma-associated herpesvirus
reactivation from latency (submitted to plos pathogens, 2010)
5) yunzhe lu, yyyyyy x. yyyyyy, rong li, and yanfen hu. brca1/bard1
complex interacts with steroidogenic factor 1----a potential mechanism for
regulation of aromatase expression by brca1 (submitted to journal of cellular
biochemistry, 2010 ).
6) yunzhe lu, yyyyyy x. yyyyyy, rong li, and yanfen hu.identification of
a novel ubiquitin e3 ligase for brca1 (manuscript in preparation)
7) marc walter,yyyyyy x. yyyyyy, rong li, and yanfen hu.regulation of
aromatase pii promoter by dna methylation
8) sagar ghosh, yyyyyy x. yyyyyy, yanfen hu, and rong li. collagen
induces aromatase expression in adipose stromal cells (manuscript in
preparation)
yyyyyy x. yyyyyy
14825 omicron drive, #704 ~ san antonio, tx 78245 ~ (xxx-xxx-xxxx ~ abc@xyz.com
date
hiring agent name
title
company name
address
city/state/zip code
dear__________________:
i am currently seeking a challenging career opportunity in a technician, lab manager, or research associate capacity and am submitting my resume for your review. in advance, thank you for your time and consideration.
as demonstrated in the accompanying resume, my professional qualifications include more than 4 years of research experience in areas of biotechnology with the university of texas at san antonio. during this experience, i have effectively applied my analytical skills and advanced knowledge of biotechnology toward research to benefit the health sciences center and the greehey children s cancer research institute.
to complement this background, i am a candidate for a master of science in biotechnology and have successfully published research in academic journals and industry articles 3 times.
as an employee, you will find me to be a driven team player committed to supporting you in achieving your objectives through superior performance. i am confident that i could be a valuable asset to your firm, and look forward to interviewing with you in the near future.
sincerely,
yyyyyy x. yyyyyy
yyyyyy x. yyyyyy
14825 omicron drive, #704 ~ san antonio, tx 78245 ~ (xxx-xxx-xxxx ~ abc@xyz.com
date
contact name
company name
address
city/state/zip code
dear _______________________________:
i would like to thank you for providing me the opportunity to interview with you and discuss the exciting (title of position applying for) position currently available with your company. having gained a deeper insight into the job requirements and your objectives, i am confident you will find me a valuable asset in achieving your goals.
as discussed, i am an enthusiastic, disciplined team player eager to launch a successful career as a dedicated member of your staff. i believe you will find my dynamic research and analytical skills as well as advanced knowledge of biotechnology applications in areas of health care and cancer research vital to your operations and look forward to contributing to your success.
should you have any additional questions, or to discuss future employment opportunities, please feel free to contact me at your earliest convenience. again, thank you for the enlightening interview. i look forward to your response.
sincerely,
yyyyyy x. yyyyyy
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