profile                                                

 

senior research associate eager to contribute expertise in academic and industrial research toward actively supporting a leading-edge biopharmaceutical firm in identifying and facilitating pioneering scientific and technological innovations to optimize quality of human lives.  offer qualifications that include, among others:

 

ø  expertise in life science, cell biology and biochemistry

ø  a decade of successful experience in animal model, primary stem cell culture and varied in vivo and vitro assays

ø  m.s. in surgery and m.d. in clinical medicine, with eight years of practical clinical experience in surgery, gynecology and obstetrics

ø  dynamic project management skills, with ability to effectively plan, coordinate and steer projects to achieve crucial timelines and goals

ø  proactive leader and collaborative team player equally effective in self-directed and managed environments

 

animal experiment background

  strong hands-on experience in a variety of animal models such as diabetic scid mice, c57bl mice and rats, acute liver failure mouse model, and acute and chronic stress models, among others.

  proficient in rodent handling including injections (ip, iv, icv and sc), surgical procedures, behavioral and pharmacological study, tissue/blood sample collections, and perfusion.

  advanced skills in cell isolation and purification from human, rat and mouse organs.

  expertise in the transplantation of cells and tissues intraperitoneally (ip) or under the kidney capsules of rats and mice (ukc); adept in macro and micro encapsulation techniques.

  solid background in glucose tolerance testing (gtt) including ogtt and ivgtt.

 

research skills

 

cell biology

  extensive experience in conducting cell culture under gmp standard; skilled in cell proliferation and differentiation.

  excel in developing, validating and performing varied cell-based assays such as cell growth viability, apoptosis assays; experience includes sgs (static glucose stimulation), facs,  magnetic cell sorting, elisa, and dna cyquant quantification, etc.

histology

  proficient in tissue and cells fixing, embedding, sectioning, he staining, immunohistochemistry (immunofluorescence and immunoperoxidase), microscopy and photography. 

molecular biology

  comprehensive background in total rna extraction, cdna synthesis, agarose gel, densitometry analysis, rt-pcr, and real time pcr.

 

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career path                                      

 

amcyte diabetes inc (acquired by reneuron at 2007), santa monica, ca                            1998-current

research scientist; focus of research: (1) isolation, in vitro expansion and characterization of human pancreatic progenitor cells. (2) human embryonic stem cell culture, differentiation and characterization

         effectively identify, select and proliferate endocrine progenitor cells from human adult pancreatic cell populations and differentiate into functional insulin-producing cells or islet-like cell clusters.

         proficiently isolate and clone differentiation medium-responsive cell populations in support of generating new cell lines.

         actively support multiple research projects by developing, optimizing and conducting a variety of cell based assays.

         hold full accountability for ensuring islet isolation and purification from rat, mouse and human pancreases is in critical compliance with sop, safety and gmp clinical manufacturing guidelines.

         perform in vitro culture of pancreatic progenitor cell in gmp environment as well as in vivo pharmacological evaluation of efficiency of islet transplantation in diabetic therapy.

         successfully develop assays to effectively evaluate ammonia detoxification and albumin secretion of cultured rat liver cells.

         steered investigation of isoforms and distribution of cell surface markers, such as ncam in human pancreatic cells.

 

cure, digestive diseases research center, dept. of medicine, ucla                                    1997-1998

research associate; focus of research: expression of a new neuropeptide pituitary adenylate cyclase activating polypeptide (pacap) receptor (paci) in gastrointestinal tract

         effectively detected expression of pac i splice variants in purified ecl cells by immunohistochemistry and rt-pcr and defined the effects of pacap on acid secretion.

 

department of general surgery, baogang third hospital, baotou, p.r. china                          1995-1997                                                                                                       

attending surgeon; research focus: effects of cholecystectomy on gastrointestinal function

         efficiently assessed duodenogastric reflex (dgr) through 24-hour continuous intragastric monitoring of ph value and gastrointestinal hormones both pre- and post-cholecystactomy.

 

department of general surgery, baogang third hospital, baotou, p.r. china                          1984-1992

resident/attending physician

         launched and built successful career in clinical practice.

education                                           

 

m.s., surgery, tianjin medical university, tianjin, p. r. china; 1995

m.d., clinical medicine, baotou medical college, baotou, p.r. china; 1984

 

patents                                              

 

  wen-ghih tsang . tianli zheng and yanping wang.  cultured and encapsulated pancreatic stem cells. international publication number wo03/059072 ai.

  wen-ghih tsang . tianli zheng and yanping wang.  in situ maturation of cultured pancreatic stem cells having a specified intermediate stage of development. us patent publication number 10/0000 xxxxxx xxxx , xxxx , xxxxx 000004-000700 us (pending).

  wen-ghih tsang . hong-jung chen. yiping tu and yanping wang.  methods of  selecting pancreatic endocrine cells using protein synthesis inhibitors. 021164-000500 us (pending).

yanping wang, md                                                                  cv-page 3 of 3                      

 

publications/abstracts                 

 

         wen-ghih tsang ,tianli zheng, yanping wang, jinghua tang, howard rind, aleksandar

francki and nataliya bufius. culture and intracapsular aggregation of adult human 

pancreatic islet tissue. transplantation. volume 83, number 6, march 27, 2007

         yanping wang, baochang lui and qingrui li. the effect of gastrointestinal hormones on

gallbladder and bile duct. guo wai yi xue endocrinology. 1996: 15(4): 211-215

         n zeng, t kang, rm luy, ga ohning, yp wang, jh wash, g sachs and jr pisegna.

pituitary adenylate cyclase activating peptide (pacap): the candidate peptide for central   

regulation of gastric and secretion. annual meeting of american gastroenterological 

association, new orleans, may 16-22, 1998.

         w.g. tsang, c.c. huntenburg, t. zheng, a. schaeffer, y.p. wang, k. lim e. leoncio and p. tram. development of encapsulated human islets: effect of material selection on product performance. nih workshop-immununilisolation, washington dc, march 29-30, 2004.

         wen-ghih tsang, tianli zheng, yanping wang and nataliya bufius.  in vitro generated

islet-like-clusters exhibit similar cellular architecture and function to cultured

encapsulated islets.  diabetes volume 53 supplement 2 :a455, 2004

         wen-ghih tsang, carolyn c. huntenburg, tianli zheng, andrew schaeffer, yanping wang

and kermit lim.  in vitro and in vivo characterization of the encapsulated islet for the clinical

trial entitled prospective phase i/ii safety study of encapsulated isolated primary human

pancreatic islet transplantation with low dose short term immune suppression in patients

with type i diabetes mellitus american transplant congress, boston, ma, may 14 c

19,2004.

         w. tsang, t. zheng, y. wang, n. bufius, h. chen. in vitro proliferation of human pancreatic islet cells and assembly into functional islet-like clusters. the 44^th ascb  annual meeting in washington dc, 2004

         hong-jung chen, wen-ghih tsang, tianli zheng, yanping wang, nataliya bufius, wen

chen, zhi-duan zhong, jinghua tang. proliferation of adult human endocrine progenitor

cells and differentiation into functional islet-like clusters. the 3^rd isscr annual meeting

in san francisco, 2005.

         hong-jung chen, yiping tu, yanping wang, jennitte stevens, aleksandar francki,

wen-ghih tsang. selection of pancreatic endocrine lineage cells using protein   

synthesis inhibitor. the 4^th isscr annual meeting in toronto, 2006

         aleksandar francki, zhi-duan zhong, wen chen, yanping wang, jinghua tang, erik pong, andrew schaeffer, wen g. tsang. expression of ncam in cultured adult human pancreatic cells delineates mature endocrine cells as well as putative progenitors. 2006, american diabetes association 66th scientific sessions, washington dc

         hong-jung chen, yiping tu, erik pong, yanping wang, wen chen, anakim chew, nataliya buffius, hui-ren liu, aleksandar franki, zhi-duan zhong, wen-ghih tsang;. proliferation and in vivo differentiation of selected adult human pancreatic endocrine progenitor cells. 2006, 7th annual rachmiel levine symposium, anaheim, ca.